Hybridoma technology for production of monoclonal antibodies: Monoclonal antibodies are produced by hybridoma technology.
Acta Oncol. The use of immuno-cytochemical techniques increases diagnostic accuracy in these cases. The fusion is done by using Polyethylene glycol PEG or by electrophoration or by using phages. Invention of the Hybridoma Technology Added by: Dr. Kohler and Milstein were awarded a share of the Nobel prize in physiology or medicine for their breakthrough.
Fused cells are incubated in HAT medium hypoxanthine - aminopterin - thymidine medium for roughly 10 to 14 days.
Monoclonal antibodies are produced against a variety of proteins, glycoproteins, glycolipids, nucleic acids and chemically defined groups linked to protein carriers. Cotton's previous experiments, as well as a publication by J.